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DGIMI est une unité mixte de recherche ayant pour tutelles INRAE et l'Université de Montpellier. Elle est située sur le campus Triolet de l'Université de Montpellier et accueille une trentaine de personnels INRAE et UM.

Les recherches développées dans l'UMR DGIMI visent à mieux comprendre les interactions entre les insectes et leur environnement biotique et abiotique dans les agrosystèmes, selon deux axes thématiques 

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HAL : Dernières publications

  • [hal-05155756] Characterization of the genomic sequence of an iflavirus, a protoambidensovirus, and of three microviruses detected in mosquitoes (Aedes albopictus and Culex quinquefasciatus)

    We report the complete CDS of five viruses: XiangYun picorna-like virus 4 ( Iflaviridae ), Protoambidensovirus dipteran1 ( Parvoviridae ), and three microviruses ( Microviridae ), detected by viromics surveillance of Aedes albopictus and Culex quinquefasciatus from Réunion Island. The protoambidensovirus detected in A. albopictus belongs to a clade previously reported only in Culex pipiens.

    ano.nymous@ccsd.cnrs.fr.invalid (Sarah François) 10 Jul 2025

    https://hal.inrae.fr/hal-05155756v1
  • [hal-05154488] Is antibiotic (mis)use an emerging ecological threat to wild insects?

    Antibiotic discovery revolutionized human healthcare and boosted agricultural productivity. The efficacy of this revolution is now being challenged however, as the release of antibiotic residues into soils and waterways promotes the evolution and spread of antibiotic resistance genes. There are signs that this antibiotic pollution also has ecological costs, including reduced insect health. Here, we assess this risk. We discuss where, when and how wild insects are exposed to antibiotics, the consequences of this exposure and crucially, if the concentrations that wild insects encounter are sufficiently high to trigger phenotypic responses. Data demonstrate that antibiotic residues reach concentrations in the field that can have phenotypic impacts in insects. These impacts include reduced health and foraging activity in pollinators, and improved survival in insect vectors of disease, demonstrating potential consequences for food-security and disease transmission. More generally, antibiotics can reduce insect stress resistance meaning that antibiotic pollution could elevate insect susceptibility to other stressors implicated in insect declines. We highlight gaps in our understanding of how antibiotic pollution affects wild insects and the ecosystem services they provide. These gaps urgently need to be filled, because global antibiotic use is rising. Crucially, we must determine how antibiotic residues in the field degrade or accumulate along food chains, and how field-realistic concentrations affect insects directly and via interactions with other environmental stressors. This will offer concrete insights into the consequences of rising antibiotic use for insects, and their broader societal and ecological impacts.

    ano.nymous@ccsd.cnrs.fr.invalid (C. Ruth Archer) 09 Jul 2025

    https://hal.inrae.fr/hal-05154488v1
  • [hal-05156027] ATAC‐seq in Emerging Model Organisms: Challenges and Strategies

    ABSTRACT The Assay for Transposase‐Accessible Chromatin with sequencing (ATAC‐seq) is a versatile and widely utilized method for identifying potential regulatory regions, such as promoters and enhancers, within a genome. ATAC‐seq has been successfully applied to a wide range of established and emerging model organisms. However, implementing this method in emerging model systems, such as arthropods, can be challenging due to several factors that influence data quality. These factors include the availability of a sufficient amount and quality of tissue or cells, the need for species‐ and tissue‐specific protocol optimization, the completeness and accuracy of the reference genome, and the quality of the genome annotation. In this article, we emphasize the key steps in the ATAC‐seq protocol that, based on our experience, have the greatest impact on data quality when adapting this method for emerging model organisms. Specifically, we discuss the importance of nuclei isolation, the incubation conditions of the Tn5 transposase, and PCR amplification of the library. Furthermore, we outline essential quality checkpoints during the bioinformatic analysis of ATAC‐seq data to assist in assessing data integrity and consistency. Given that many emerging model organisms may not be readily available in laboratory cultures, we also emphasize the importance of evaluating how different preservation methods affect ATAC‐seq data quality. Based on examples in one spider and one ant species, we demonstrate that replication and thorough quality controls at all steps of the protocol and data analysis are essential to assess the usability of ATAC‐seq data. Our data highlights the importance of isolating the right number of intact nuclei, as well as ensuring optimal amplification conditions during library preparation to obtain good‐quality sequence data for downstream analyses. We recommend using fresh tissue samples if possible because we show that direct cryopreservation of the tissue may affect chromatin integrity. This effect could be avoided or reduced by preserving the homogenate in cell culture medium. Overall, we explain the ATAC‐seq protocol and downstream analyses in detail and give step‐by‐step advice to researchers who are new to the field and want to implement this method. With careful planning and validation, ATAC‐seq can reveal the regulatory landscape of a genome and aid in identifying elements that govern gene expression.

    ano.nymous@ccsd.cnrs.fr.invalid (Duğçar Ebrar Erdoğan) 10 Jul 2025

    https://hal.inrae.fr/hal-05156027v1

Actualités

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article

06 mars 2025

Rédaction : A-N Volkoff, N. Nègre , I. Seninet

Nouvelle publication scientifique

Les populations invasives au Sénégal montrent des traces d’évolution adaptative sur des gènes CYP450 pouvant expliquer leur adaptation au maïs.
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photo Peter Heeling

Site web en cours de maintenance

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Des modifications de structure et de contenus s'opèrent actuellement sur le site web DGIMI

Postdoctoral Position in CRISPR/Cas9 Gene Editing in Insects (University of Montpellier)

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Location: UMR-DGIMI, University of Montpellier, France / Duration: 18 months / Start Date: Preferably from April 2025

News & Views sur l’écologie des viromes d’insectes

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Un commentaire critique de Sarah François (https://www.nature.com/articles/s41559-024-02385-w), chercheuse INRAE à DGIMI, sur un article à fort impact portant sur l'écologie des communautés virales (i.e. viromes) d'insectes.